The second case of H5N8 highly pathogenetic avian influenza in the Czech republic was diagnosed on a farm in the Pardubice region. The outbreak will require culling of 140,000 chickens and some turkeys. Appropriate responses framed in terms of World Organization for Animal Health (OIE) have been implemented. The Pardubice region is in the north central quadrant of the Czech Republic and shares a common border with Poland. 