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 Using funding provided by USPOULTRY, Dr. Maricarmen Garcia of the University of Georgia applied MinION Nanopore sequencing to distinguish among genomic types of infectious laryngotracheitis virus.
From July 2023 through March 2024, 100 samples from 90 field outbreaks were genome-typed using a multiplex PCR MinION sequencing assay. Of the 97 samples that were evaluated, 78 were identified as genome type (GT) VI. Nineteen samples were classified as GT IV, corresponding to chick embryo origin vaccine, previously responsible for widespread field outbreaks.
The PCR MinION assay will add to the knowledge of ILT epidemiology and will contribute to developing appropriate prevention strategies including biosecurity and immunization.
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